- Checkpoint Mutants
- mad2∆
- bub2∆
- mad2∆ bub2∆
- cdc20-ts mad2∆
- cdc20-ts bub2∆
- WT in nocodazole
- mad2∆ in nocodazole
- mad2∆ GAL-TEM1 in nocodazole
- mad2∆ pds1∆ in nocodazole
- bub2∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- bub2∆ mad2∆ in nocodazole
- pds1∆ in nocodazole
- net1-ts in nocodazole
bub2∆ mad2∆ in nocodazole
debug: ,
test user =
test db =
Simulation:
Change of parameters: kbub2=0, kmad2=0.01, ksspn=0, init BUB2=0.
Arrest: Mitotic exit at t=105.4 min (like wild type in the absence of nocodazole)
Experiments:
Alexandru, G., Zachariae, W., Schleiffer, A. and Nasmyth, K. (1999). Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J. 18:2707-2721.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Figs. 6C and 9A, spindle-assembly checkpoint is defective. Kinetics of Clb2 is similar to that of wild type in the absence of nocodazole.
Comments:
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